As negative controls for DRD2 immunoreaction, the slides were incubated with antibodies preabsorbed with the synthetic peptide that included the immunogenic region used to produce the antibody. The inset in B corresponds to a negative control in the absence of primary antibody. A similar change in the localization pattern of tyrosine phosphorylated proteins was described previously in pig spermatozoa incubated in noncapacitation and capacitation media [ 23 ]. Capacitation of mouse spermatozoa, II: Taken together, our results support the idea that dopamine may have a fundamental role during sperm capacitation and motility in situ in the female upper reproductive tract. The inset at Time 0 corresponds to a negative control in the absence of primary antibody for both immunoreaction analyses.
In summary, dopamine seems to act as a physiological modulator of viability, capacitation, and sperm motility.
Biology of Reproduction
Stimulation of in vitro activation and the acrosome reaction of hamster spermatozoa by catecholamines. A total of spermatozoa, including all groups, were analyzed using this protocol. Mammalian sperm translate nuclear-encoded proteins by mitochondrial-type ribosomes. Two to three separate fields were taken for each sample. After repeated washing of the slide in bidistilled water, the spermatozoa were analyzed using epifluorescence Zeiss Axioplan 2 microscope; Carl Zeiss Gmbh, Jena, Germany. Further evidence suggesting the hormonal stimulation of hamster sperm acrosome catecholamines in vitro.